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本文建立了一种反相高效液相色谱-紫外光谱检测法,可同时定量检测维力波糖、米格列醇、维格列波糖和阿卡波糖这4种α-葡糖苷酶抑制剂。采用Prevail carbohydrate(250mm×4.6mm,5μm)色谱柱,以乙腈-磷酸盐缓冲液(pH8.0)进行梯度洗脱,在210nm下进行检测,结果维力波糖、米格列醇、维格列波糖和阿卡波糖的线性范围分别为58.2～932.0μg·mL-1(r=0.9999,n=5)、23.5～376.0μg·mL-1(r=0.9999,n=5)、0.128～2.050mg·mL-1(r=0.9999,n=5)、38.2～612.0μg·mL-1(r=0.9999,n=5);平均回收率分别为97.0%、103.6%、96.4%和100.4%(n=9)。与此同时,本文还建立高相液相色谱-质谱检测方法,用于这4种化合物的定性分析。以上两种方法简便快捷,结果准确可靠、重复性好,可用于可疑的糖尿病药品中非法添加α-葡糖苷酶抑制剂的定性定量检测。
In this paper, a reversed-phase high performance liquid chromatography-ultraviolet spectrophotometry was developed to detect the simultaneous inhibition of four α-glucosidase inhibitors, including glyburide, miglitol, voglibose and acarbose Agent. Prevail carbohydrate (250 mm × 4.6 mm, 5 μm) column was used for gradient elution with acetonitrile-phosphate buffer (pH 8.0) and the detection was carried out at 210 nm. The results showed that all of the glyburide, miglitol, The linear ranges of leptin and acarbose were 58.2-932.0 μg · mL -1 (r = 0.9999, n = 5), 23.5-376.0 μg · mL -1 (r = 0.9999, n = 5) The average recoveries were 97.0%, 103.6%, 96.4% and 100.4 respectively (r = 0.9999, n = 5) and 38.2-612.0 μg · mL -1 % (n = 9). In the meantime, high-performance liquid chromatography-mass spectrometry (HPLC-MS) was also developed for the qualitative analysis of these four compounds. The above two methods are simple and quick, the results are accurate and reliable, and the repeatability is good. The method can be used for qualitative and quantitative detection of illegally added α-glucosidase inhibitors in suspected diabetic drugs.