论文部分内容阅读
目的:探讨β-石竹烯(β-Caryophyllene)对脑缺血再灌注大鼠的保护作用及其机制。方法:将75只SD大鼠随机分为5组:假手术组(Sham);模型组(I/R);β-石竹烯34mg/kg组;β-石竹烯102mg/kg组;β-石竹烯306mg/kg组。采用线栓法建立大鼠大脑中动脉栓塞(Middle Cerebral Artery Occlusion,MCAO)模型,缺血1.5h,再灌注24h后,采用双盲法进行神经行为学评分;尼氏染色法(Nissl)测定大脑皮质神经元缺损情况;原位末端凋亡法(TUNEL)检测皮质神经元凋亡情况;测定皮质中超氧化物歧化酶(SOD)、丙二醛(MDA)含量;Western法检测核因子相关因子2(NF-E2-related factor,Nrf2)及血红素氧合酶1(heme oxygenase 1,HO-1)表达。结果:β-石竹烯102mg/kg、306mg/kg剂量范围内可显著改善MCAO大鼠神经缺损功能;减轻皮质神经元缺损及细胞凋亡情况;上调SOD水平,降低MDA含量;上调Nrf2、HO-1蛋白的表达。结论:β-石竹烯对脑缺血再灌注大鼠具有保护作用,此作用可能与其抗氧化作用有关。
Objective: To investigate the protective effect of β-Caryophyllene on cerebral ischemia-reperfusion in rats and its mechanism. Methods: Seventy-five Sprague-Dawley rats were randomly divided into 5 groups: Sham model group (I / R), β-Caryophyllene 34mg / kg group Alkene 306 mg / kg group. The rat model of middle cerebral artery occlusion (MCAO) was established by the method of thread embolism. The neurobehavioral score was measured by double-blind method after 1.5h of ischemia and 24h after reperfusion. Nissl staining Cortical neuron defects were detected by immunohistochemistry. Apoptotic cortical neurons were detected by TUNEL. The content of cortical superoxide dismutase (SOD) and malondialdehyde (MDA) was determined. The levels of nuclear factor-kappa2 (NF-E2-related factor, Nrf2) and heme oxygenase 1 (HO-1). RESULTS: The dose of 102 mg / kg and 306 mg / kg of β-Caryophyllene could significantly improve the neurological deficits, alleviate the neuronal defects and apoptosis of MCAO rats, upregulate the level of SOD, decrease the content of MDA, upregulate the expression of Nrf2 and HO- 1 protein expression. CONCLUSION: β-Caryophyllene has a protective effect on cerebral ischemia-reperfusion rats, which may be related to its anti-oxidative effects.