用0.3％的果胶酶和0.3％的纤维素酶处理锦橙(Citrus sinensis Osbeck)胚性愈伤组织16小时,得到91.2％具有活性的原生质体。培养4天时,出现第一次细胞分裂,二个月时,再生出胚状体。胚状体转入加有赤霉素和硫酸腺嘌呤的培养基中一个月,再生成完整的植株。原生质体再生出的胚状体中,没有发现有染色体数目的变异。锦橙非胚性愈伤组织的原生质体能分裂形成细胞团,但没有再生出植株。对这种原生质体的再生和利用作了讨论。 Citrus sinensis Osbeck embryogenic calluses were treated with 0.3% pectinase and 0.3% cellulase for 16 hours to yield 91.2% active protoplasts. When cultured for 4 days, the first cell division occurred and embryos were regenerated two months later. Embryoid bodies are transferred to medium supplemented with gibberellin and adenine sulphate for one month to regenerate intact plants. Protoplasts were regenerated from embryoid bodies, and no variation in the number of chromosomes was found. Protoplasts from the non-embryogenic callus of C. sinensis were able to divide to form cell clusters but no regenerated plants. This protoplast regeneration and utilization are discussed.