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以祁连山青海云杉叶片为研究对象,通过测定叶片稳定碳同位素比率(δ13C)、叶片含水量(LWC)、脯氨酸含量、灰分含量、碳含量、氮含量、碳氮比、土壤含水量(SWC)等指标,探讨不同水分梯度下叶片δ13C的控制因子.结果表明,祁连山青海云杉叶片δ13C值在-28.9‰～-25.4‰之间变化,平均值为-27.3‰.沿河西走廊从东到西的干旱梯度(降水量逐渐减少),叶片δ13C表现出偏正的趋势.叶片δ13C与LWC和脯氨酸含量呈显著正相关关系,与灰分含量、SWC显著负相关.叶片δ13C值在青海云杉种内分布稳定,主要是由自身的遗传特性所决定,同时与生长环境条件也有一定的关系,其中土壤可利用水分是最主要的限制因子.叶片灰分含量是δ13C值的可选择性替代指标.
In this paper, the Qingshan Mountains Qinghai spruce leaves were studied by measuring the stable carbon isotope ratio (δ13C), leaf water content (LWC), proline content, ash content, carbon content, nitrogen content, carbon and nitrogen ratio, soil water content SWC) were used to study the control factors of leaf δ13C in different water gradients.The results showed that the δ13C value of spruce leaves varied from -28.9 ‰ to -25.4 ‰ in Qilian Mountains, with an average value of -27.3 ‰, Δ13C in leaves showed a positive trend.The δ13C of leaves showed a significant positive correlation with LWC and proline content, but negatively correlated with ash content and SWC.The leaf δ13C value was in the range of The distribution of Picea crassifolia was stable within the species, which was mainly determined by its own genetic characteristics and also had some relations with the growth environment conditions, of which the soil available water was the most important limiting factor.The leaf ash content was the selectivity of δ13C value Alternative indicators.