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目的探讨慢性重型肝炎与隐孢子虫感染的关系,为防治该病提供依据。方法用金胺-酚染色法(AA-P)和改良抗酸染色法(MAF)检测218例慢性重型乙型肝炎(慢重肝)患者和140例腹泻儿童粪便标本隐孢子虫卵囊,用PCR技术检测其DNA,并进行内切酶分析;对隐孢子虫感染影响因素进行分析。结果经AA-p、MAF和PCR检测,慢重肝患者隐孢子虫感染率分别为4.1%、3.2%和6.0%;腹泻儿童感染率为0.7%、0.7%和1.4%。PCR结果显示,慢重肝患者隐孢子虫感染率高于腹泻儿童;阳性患者腹泻史、动物接触史明显高于阴性患者,农村患者感染率高于城市患者。结论慢性重型肝炎患者对隐孢子虫的易感性增加,隐孢子虫感染是引起慢性重型肝炎患者腹泻和可能加重其病情的因素之一。
Objective To investigate the relationship between chronic severe hepatitis and Cryptosporidium infection and provide basis for the prevention and treatment of this disease. Methods Cryptosporidium oocysts in 218 cases of chronic severe hepatitis B (chronic severe hepatitis) and 140 cases of diarrhea children were detected by gold amine-phenol staining (AA-P) and modified acid-fast staining (MAF) PCR detection of DNA, and endonuclease analysis; analysis of the impact of Cryptosporidium infection factors. Results The infection rates of Cryptosporidium in patients with chronic severe hepatitis were 4.1%, 3.2% and 6.0% respectively by AA-p, MAF and PCR. The infection rates of children with diarrhea were 0.7%, 0.7% and 1.4% respectively. PCR results showed that the prevalence of Cryptosporidium in patients with chronic severe hepatitis was higher than that in children with diarrhea; the history of diarrhea and the history of animal contact in positive patients were significantly higher than those in negative patients, and the prevalence in rural areas was higher than that in urban patients. Conclusion The susceptibility to Cryptosporidium in patients with chronic severe hepatitis is increased. Cryptosporidium infection is one of the factors that cause diarrhea and may aggravate its condition in patients with chronic severe hepatitis.