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本文研究了中国木薯栽培种四种外植体通过器官发生再生植株的条件。结果表明:在MS附加0.05mg/L TIBA,1mg/L BA的培养基上“NZ 188”初步的萌发胚状体“切头”后切口处可直接产生丛芽,出芽率为43%。“SC201”胚状体子叶块在MS附加0.5 mg/L NAA,0.5mg/L BA的培养基上可直接出芽,出芽率为42%,在MS附加0.5mg/L IBA,1.5mg/L BA培养基上·出芽率为31%,AgNO_3和ABA单独使用或配合使用均不利于芽的再生。“NZ188”胚状体下胚轴在MS附加0.5mg/LNAA,0.5mg/L BA的培养基上形成的愈伤组织转入MS附加1mg/L NAA,2mg/L BA的培养基上,3周后大多数愈伤组织有绿点出现、仅4.4%外植体分化出芽。“HZ188”无菌苗茎段接种在MS附加0.05mg/L TIBA,2mg/LBA的固体培养基上,2周后形成大量愈伤组织,4周后仅见一块愈伤组织分化出芽。
In this paper, we studied the regeneration of four explants from Chinese cassava cultivars through organogenesis. The results showed that in the medium of MS supplemented with 0.05 mg / L TIBA and 1 mg / L BA, the shoots of the “NZ 188” initial germinating embryo “incision” could directly produce the clustered shoot with the germination rate of 43%. The embryos of “SC201” embryos could directly germinate on the medium supplemented with 0.5 mg / L NAA and 0.5 mg / L BA, the germination rate was 42%, and 0.5 mg / L IBA and 1.5 mg / L BA The budding rate was 31% on the medium. AgNO_3 and ABA alone or in combination did not help bud regeneration. The embryogenic hypocotyls of “NZ188” were transformed into MS supplemented with 1 mg / L NAA and 2 mg / L BA on the medium supplemented with 0.5 mg / L NAA and 0.5 mg / L BA. 3 Most of the callus appeared green spots after week, with only 4.4% explants budding. Aseptic shoots of “HZ188” were inoculated on solid medium supplemented with 0.05mg / L TIBA and 2mg / LBA MS. After 2 weeks, a large amount of callus was formed, and only one piece of callus appeared budding after 4 weeks.