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目的观察白蒺藜有效组分对自发性高血压大鼠心肌结缔组织生长因子(CTGF)表达的影响,探讨白蒺藜有效组分改善心室重构的可能作用机制。方法 32只14周龄雄性自发性高血压大鼠随机分为模型组、卡托普利组、白蒺藜组、联用组,每组8只,同周龄雄性Wistar大鼠8只作为正常对照组。用药组分别给予卡托普利30mg.kg-1.d-1,白蒺藜有效组分15mg.kg-1.d-1,白蒺藜有效组分15mg.kg-1.d-1+卡托普利30mg.kg-1.d-1,模型组及对照组采用等量0.9%NaCl溶液,连续灌胃8周。采用尾动脉容积法测定血压;分离左心室,透射电镜观察心肌超微结构;RT-PCR法检测心肌转化生长因子β1(TGFβ1)、CTGF mRNA含量变化。结果与Wistar大鼠比较,14周龄SHR大鼠收缩压明显升高(P<0.01)。8周后,模型组大鼠收缩压明显较14周龄时明显升高(P<0.05),通过透射电镜对心肌超微结构观察提示,22周龄SHR出现明显的心肌纤维化;且模型组TGFβ1及CTGF mRNA较正常组表达明显增加(P<0.05)。与模型组比较,白蒺藜组收缩压明显下降(P<0.01),并可明显改善心肌超微结构,显著下调TGFβ1及CTGF mRNA水平(P<0.05)。卡托普利和白蒺藜联用可明显下调TGFβ1mRNA水平(P<0.05)。结论自发性高血压大鼠心室肌TGFβ1及CTGF表达明显增加,白蒺藜有效组分可显著抑制TGFβ1及CTGF基因表达水平,从而保护高血压病所致的靶器官损伤。
Objective To observe the effect of effective components of Tribulus terrestris on the expression of connective tissue growth factor (CTGF) in spontaneously hypertensive rats and to explore the possible mechanism of the effective components of Tribulus terrestris to improve ventricular remodeling. Methods Thirty-two male 14-week-old spontaneously hypertensive rats were randomly divided into model group, captopril group, Tribulus terrestris group and combination group, with 8 rats in each group and 8 male Wistar rats of the same age as normal control group. The treatment groups were given captopril 30mg.kg-1.d-1, Tribulus terrestris active ingredients 15mg.kg-1.d-1, Tribulus terrestris active ingredients 15mg.kg-1.d-1 + Cato Puli 30mg.kg-1.d-1, model group and control group using the same amount of 0.9% NaCl solution, continuous gavage for 8 weeks. The left ventricle was isolated and the ultrastructure of myocardium was observed by transmission electron microscopy. The content of transforming growth factor-β1 (TGFβ1) and CTGF mRNA in myocardium was detected by RT-PCR. Results Compared with Wistar rats, systolic blood pressure of 14-week-old SHR rats increased significantly (P <0.01). After 8 weeks, the systolic pressure of the model group was significantly higher than that of the 14th week (P <0.05). Ultrastructural observation of the myocardium by transmission electron microscopy showed that myocardial fibrosis was observed in the 22-week-old SHR. The expression of TGFβ1 and CTGF mRNA in normal group were significantly increased (P <0.05). Compared with the model group, the contractile pressure of Tribulus terrestris decreased significantly (P <0.01), and myocardial ultrastructure was significantly improved, and the levels of TGFβ1 and CTGF mRNA were significantly decreased (P <0.05). Captopril and Tribulus terrestris combined can significantly reduce TGFβ1 mRNA levels (P <0.05). Conclusion The expressions of TGFβ1 and CTGF in ventricular muscle of spontaneously hypertensive rats are significantly increased. The effective components of Tribulus terrestris can significantly inhibit the gene expression of TGFβ1 and CTGF and thus protect the target organs induced by hypertension.