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为发掘对蛴螬有防治作用的苏云金芽孢杆菌及其cry基因,在河北省各县市共采集土壤样品254份,采用温度筛选法分离出Bt菌株42株,经PCR-RFLP方法鉴定,其中10株含有cry8型基因。这些菌株有如下特点:同一菌株含1种或多种质粒;PCR-RFLP鉴定证明有多种cry8基因存在,多数菌株中含2种cry8基因,如cry8E和cry8F常共存于同一菌株中;Cry8杀虫晶体蛋白分子量约为130~140 k Da,晶体形态均为球形。这10株菌采样地点分布于保定、衡水、邯郸、廊坊、唐山和邢台等地,植被类型多样化,说明cry8型Bt菌株在河北省分布广泛,可为蛴螬类害虫的防治提供丰富的Bt资源。
In order to explore Bacillus thuringiensis and its cry gene, which have control effect on Canker, 254 samples of soil samples were collected in all counties of Hebei Province. 42 strains of Bt strains were isolated by temperature screening and identified by PCR-RFLP, of which 10 Contains cry8 type gene. These strains have the following characteristics: the same strain contains one or more plasmids; PCR-RFLP identification of a variety of cry8 genes exist, most of the strains contain two kinds of cry8 genes, such as cry8E and cry8F often co-exist in the same strain; Cry8 kill Insect crystal protein molecular weight of about 130 ~ 140 kDa, crystal morphology are spherical. The 10 strains were located in Baoding, Hengshui, Handan, Langfang, Tangshan and Xingtai, with diversified vegetation types, indicating that cry8 Bt strains are widely distributed in Hebei Province and can provide abundant Bt resources for the prevention and control of sufferers .