随机突变筛选是一个耗费时间和资源但十分有效的传统手段。采用重组DNA技术的代谢工程为提高发酵效价提供了一个新颖的理性化筛选方法,对于工业中传统的经验方法是一个很好的补充。通过对掌控次级代谢产物生产的特异调控系统进 Random mutation screening is a time-and resource-efficient but traditional method. Metabolic engineering using recombinant DNA technology provides a novel rational screening method for increasing the titer of fermentation, which is a good complement to the traditional empirical methods in industry. Through the control of secondary metabolites production-specific regulation system into