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A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M2 mutant pool mutagenized by ~(60)Coγ-ray.Compared with wild type plants,Ose9 1 developed longer empty glumes and rudimentary glumes.In some Oseg 1 mutants,the number of stamens of flowers was reduced and leaf-like lodicules occurred,and excessive lemma/palea-like organ could be observed in some mutant spikelets.This indicated that OsEG1 could regulate the development of rudimentary glumes,empty glumes,lemma/palea,lodicules,and stamens.Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus.To clone OsEG1 gene,F_2 population was constructed by a cross between Ose9 1(Japonica)and Guangluai4(Indica).Using map-based cloning approach,OsEG1 was mapped on chromosome 4,between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm,respectively.These results are useful for further cloning and functional analysis of the OsEG1 gene.
A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M2 mutant pool mutagenized by ~ (60) Co gamma-ray. Compared with wild type plants, Ose 9 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma / palea-like organ could be observed in some mutant spikelets. This indicates that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma / palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F_2 population was constructed by a cross between Ose9 1 (Japonica) and Guangluai4 (Indica) .Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gen e.