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目的探讨哮喘豚鼠肺泡表面活性物质分泌的影响,为哮喘的防治提供实验依据。方法卵蛋白致敏复制哮喘豚鼠模型,用高效液相色谱法测定各组豚鼠支气管肺泡灌洗液(Broncho-alveolar lavage Fluid,BALF)中磷脂成分的含量。结果哮喘豚鼠肺表面活性物质主要活性成分磷脂酰胆碱(Pc)、磷脂酰乙醇胺(Pe)的含量正常对照组显著降低,中药循经敷贴后能显著升高Pc、Pe的含量,与生理盐水、地塞米松各组比较差异均有统计学意义(P<0.05)。结论中药敷贴对哮喘有治疗作用,明显促进肺表面活性物质的磷脂分泌可能为其作用机制之一。
Objective To investigate the effects of alveolar surfactant secretion in asthmatic guinea pigs and provide experimental evidence for the prevention and treatment of asthma. Methods Asthmatic guinea pigs were sensitized with ovalbumin and the content of phospholipid in bronchoalveolar lavage fluid (BALF) of guinea pigs was determined by high performance liquid chromatography (HPLC). Results The content of phosphatidylcholine (Pc) and phosphatidylethanolamine (Pe) in lung surfactant of asthmatic guinea pigs were significantly lower than those in normal control group Saline, dexamethasone differences were statistically significant (P <0.05). Conclusion Traditional Chinese medicine has a therapeutic effect on asthma and obviously promotes the phospholipid secretion of pulmonary surfactant, which may be one of its mechanisms.