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目的:探讨建立检测板蓝根颗粒消炎成分的高效液相色谱法(HLPC法)。方法:利用HLPC检测板蓝根颗粒中消炎成分腺苷的含量,色谱条件:采用Waters Symmetry C18液相色谱柱(150×4.6mm,5μm),流动相为甲醇∶醋酸∶水(10∶10∶80)进行梯度洗脱,流速为1.2m L/min,柱温为30℃,检测波长为260nm,进样量为15μL。结果:板蓝根颗粒中腺苷进样量与峰面积线性关系良好(r=0.9998)的范围为0.0250~0.1550μg,精密度试验中RSD为0.78%、重现性试验中RSD为0.69%;平均加样回收率为99.54%(RSD=0.82%);腺苷含量为0.0700mg/g(RSD=0.78%)。结论:HLPC检测板蓝根颗粒消炎成分的方法精密度、重现性良好,操作简便、快捷,测定结果准确,可作为板蓝根颗粒制剂质量控制标准的检测方法。
Objective: To establish a high performance liquid chromatography (HLPC method) for the determination of anti-inflammatory components of Banlangen granules. Methods: The content of adenosine, an antiinflammatory component in Banlangen granule, was determined by HLPC. The chromatographic conditions were as follows: the mobile phase consisted of methanol: acetic acid: water (10:10:80) with a Waters Symmetry C18 column The gradient elution was carried out at a flow rate of 1.2 m L / min with a column temperature of 30 ° C and a detection wavelength of 260 nm. The injection volume was 15 μL. Results: The linear relationship between the concentration of adenosine in Banlangen granules and peak area was good (r = 0.9998) in the range of 0.0250 ~ 0.1550μg, RSD in precision test was 0.78%, RSD was 0.69% in reproducibility test. The sample recovery was 99.54% (RSD = 0.82%); the adenosine content was 0.0700 mg / g (RSD = 0.78%). Conclusion: The method of HLPC for the anti-inflammatory component of Radix isatidis has good precision and reproducibility. The method is simple, rapid and accurate. It can be used as the quality control standard of Banlangen Granules.