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目的 应用聚合酶链反应,检测人乳头状瘤病毒(Humanpapillomavirus,HPV)基因与结肠癌及癌区周边组织的相关性。方法 将结肠镜检获取的72例活检标本进行病理检测,其中结肠癌46例,非癌26例(结肠癌周边组织标本10例),标本用蜡块包埋与固定液两种方法固定,用聚合酶链反应(PCR)特定DNA片段体外扩增法。结果 结、直肠癌人乳头状瘤毒基因检测阳性率434%,结、直肠癌周边组织阳性率10%,非癌组织阳性率为0%。结论 癌区组织基因(HPVs)检测率较高,与非癌对照组相比,差异有显著性(P<0.05),癌组织中HPVs主要以HPV1633型和HPV18型为主,统计学分析表明结、直肠癌的发生、发展与HPV感染有一定的相关性,尤以HPV16型关系更为密切。
OBJECTIVE: To detect the association between human papillomavirus (HPV) gene and colon cancer and peripheral tissues of the cancerous region by polymerase chain reaction. Methods Seventy-two biopsy specimens collected by colonoscopy were examined pathologically, 46 of which were colon cancer and 26 were non-cancerous (10 specimens of colon cancer tissues). The specimens were fixed with wax block and fixed solution. Polymerase chain reaction (PCR)-specific DNA fragment in vitro amplification method. Results The positive rate of human papillomavirus gene detection was 344% in colorectal cancer and rectal cancer. The positive rate in peripheral tissues of colorectal cancer and rectal cancer was 10%. The positive rate in non-cancer tissue was 0%. Conclusion The detection rate of HPVs in cancer tissue was higher than that in non-cancerous control group (P<0.05). HPVs in cancer tissues were mainly HPV1633 and HPV18. Statistical analysis showed that The occurrence and development of rectal cancer have a certain correlation with HPV infection, especially with the HPV16 type.