论文部分内容阅读
以诸葛菜组织培养中产生的不定芽为繁殖体,海藻酸钠为包埋基质,添加纳米TiO_2制作人工种皮,观察添加不同浓度的纳米TiO_2对诸葛菜人工种子萌发和抗菌能力的影响,将纳米TiO_2与NAA以及多菌灵的作用进行对比并研究共同作用。结果表明:纳米TiO_2具有与NAA相似的生长素作用,并且显著提高萌发生根速度,二者具有协同作用;无菌条件下0.1mg·L~(-1) NAA+30g·L~(-1) TiO_2+1/2MS+3%蔗糖的组合使诸葛菜人工种子萌发率达到100%,生根率达到80%,为最佳组合;有菌条件下添加30g·L~(-1)纳米TiO_2试验组15d污染率仅为5.6%,萌发率达83.33%,为最佳组合。纳米TiO_2和多菌灵混合作用反而使萌发率和抗菌能力大幅降低。
Adopting adventitious buds produced from tissue culture of Brassica campestris as the propagule and sodium alginate as the embedded matrix, the artificial seed coat was made by adding nano-TiO_2. The effects of adding different concentrations of nano-TiO_2 on the germination and antibacterial capacity of the artificial seeds were observed. The effect of nano-TiO 2 with NAA and carbendazim is compared and studied. The results showed that nano-TiO_2 had auxin-like effect similar to NAA and significantly increased the rate of germination and rooting, both of which had a synergistic effect. Under the condition of 0.1 mg · L -1 NAA and 30 g · L -1, The combination of TiO_2 + 1 / 2MS + 3% sucrose resulted in 100% germination rate and 80% rooting rate of Artemisia selengensis L. seeds, and 30 g · L -1 TiO_2 group 15d pollution rate of only 5.6%, germination rate of 83.33%, the best combination. Nano-TiO 2 and carbendazim mixed effect but the germination rate and antibacterial capacity significantly reduced.