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目的:研究微量元素锶对幼年自发性高血压大鼠(Spontaneously hypertensive rats,SHR)血压升高的预防作用并探讨其作用机制。方法:107只自发性高血压大鼠适应性喂养1周后,随机分为A组纯水对照组22只、B组锶水浓度3mg/L组22只、C组锶水浓度9mg/L组19只、D组锶水浓度18mg/L组22只,E组锶水浓度36mg/L组22只,普通饲料喂养12周。实验开始时测定基础血压,至结束时共测十次血压。实验开始及结束采血两次,检测肝、肾功能,Elisa法检测血清内皮素-1(endothelin-1,ET-1)、肾素(renin)、血管紧张素I(Iangiotensin-II)及去甲肾上腺素(noradrenalin,NA),免疫组化法检测主动脉核因子κB(nuclear factor-kappa B,NF-κB)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)、细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)的表达。结果:(1)SHR成年后,E组与A组相比表现出血压下降趋势(P<0.05);第十、十一、十二周时D、E组血压均显著低于A组(P<0.01);B、C组较A组也有不同程度下降;随锶水浓度增高血压呈逐渐下降的大体趋势,B-E组血压值低于A组(P<0.05,P<0.01);(2)锶水喂养后各组肝、肾功能指标及ET-1、renin、Ang-II、NA变化无明显差异(P>0.05);(3)锶水喂养后,B-E组主动脉NF-κB、VCAM-1;ICAM-1的积分光密度值(IOD)均显著低于A组(P<0.01),且随锶水浓度增高,NF-κB、VCAM-1、ICAM-1的表达呈递减趋势(P<0.01)。结论:微量元素锶可延缓SHR血压升高,减小血压波动,其对高血压的预防作用机制可能是由于抑制NF-κB经典通路,减少VCAM-1、ICAM-1等炎症介质的产生而减轻血管壁损害。
Objective: To study the preventive effect of trace element strontium on the blood pressure in juvenile spontaneously hypertensive rats (SHR) and to explore its mechanism. Methods: One hundred and seven spontaneously hypertensive rats were fed one week after their adaptive feeding. They were randomly divided into two groups: control group A, control group 22, group B, strontium water 3mg / L, group C, strontium water 9mg / 19, D group strontium water concentration 18mg / L group 22, E group strontium water concentration 36mg / L group 22, normal feed 12 weeks. The basal blood pressure was measured at the beginning of the experiment, and the blood pressure was measured ten times by the end of the experiment. The blood samples were collected at the beginning and the end of the experiment. The liver and kidney function were examined. The serum levels of endothelin-1 (ET-1), renin, Iangiotensin-II, (Noradrenalin, NA) and immunohistochemistry were used to detect the expression of nuclear factor-kappa B (NF-κB), vascular cell adhesion molecule-1 (VCAM- Intercellular adhesion molecule-1 (ICAM-1) expression. Results: (1) Compared with group A, group E showed a trend of decreasing blood pressure (P <0.05) after SHR adult; at the tenth, eleventh and twelfth week, the blood pressure of group D and E were significantly lower than that of group A <0.01). The blood pressure in BE group was lower than that in A group (P <0.05, P <0.01); (2) There was no significant difference in the indexes of liver and kidney, ET-1, renin, Ang-II and NA in each group after feeding with strontium water (P> 0.05); (3) -1, ICAM-1 were significantly lower than that of group A (P <0.01), and the expression of NF-κB, VCAM-1 and ICAM-1 decreased with the increase of strontium concentration P <0.01). CONCLUSIONS: Trace element strontium can delay SHR blood pressure and decrease blood pressure fluctuation. Its preventive effect on hypertension may be due to inhibition of NF-κB classical pathway and reduction of inflammatory mediators such as VCAM-1 and ICAM-1 Blood vessel wall damage.