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棉酚由于其特定的结构而不溶于水,微溶于醇,属脂溶性质,因而在实验研究中(如作静脉注射、离体细胞培养等),脂溶性棉酚溶液显然不能满足要求。为此,我们摸索了棉酚水溶液的制备方法,经抗生育和对离体细胞效应的检验,效果良好。一、方法将重蒸馏水煮沸2小时,除去溶解于水中的氧,供配制试剂用。称取醋酸棉酚7.0克(上海粮油工业公司油脂研究室)于三颈烧瓶中,加250毫升乙醚(用硫酸亚铁去除过氧化物并重蒸)溶解,然后通入氮气,同时用导管从三颈烧瓶中导出余气,使反应在通氮气条件下进
Because of its specific structure, gossypol is insoluble in water, slightly soluble in alcohol, and is a fat-soluble substance. Therefore, in the experimental research (such as intravenous injection, in vitro cell culture, etc.), the gossypol solution obviously can not meet the requirements. To this end, we explored the preparation of gossypol aqueous solution, after anti-fertility and test the effect of isolated cells, the effect is good. First, the method will boil distilled water for 2 hours to remove the dissolved oxygen in water for the preparation of reagents. Weigh acetic gossypol 7.0 grams (Shanghai Oil Industry Company Oil Research Laboratory) in a three-necked flask, add 250 ml of ether (with ferrous sulfate to remove the peroxide and re-steam) dissolved, and then purged with nitrogen from the catheter three Remaining gas was drawn from the neck flask and the reaction was allowed to run under nitrogen