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我们研究了依据丙型肝炎病毒核酸5’端高度保守的非编码区(NC)序列设计的“巢式”引物,建立了检侧血清标本中丙型肝炎病毒核酸的“巢式”聚合酶链式反应技术。经血清丙肝病毒RNA的提取,CONA合成,使用“巢式”引物的聚合酶链式反应三个步骤,扩增产物经溴化乙染色紫外灯下观察,对40例临床确诊非甲非乙型肝炎忠者,10例慢性型开肝炎患者和10例正常献血员血清进行了丙肝病毒RNA检测,同时进行了血清抗-HCV检测。结果表明该方法敏感及特异性强,可应用于丙型肝炎的临床早期诊断及献血员筛选。
We studied a “nested” primer designed based on the highly conserved noncoding region (NC) sequence at the 5 ’end of the Hepatitis C virus nucleic acid and established a “nested” polymerase chain to detect hepatitis C virus nucleic acids in sera Reaction Technology. Through the three steps of extraction of serum hepatitis C virus RNA, CONA synthesis and polymerase chain reaction using “nested” primer, the amplified product was observed under UV light of ethidium bromide staining, and 40 cases of clinically diagnosed non-A non-B type Hepatitis Zhongzhong, 10 cases of chronic hepatitis and 10 cases of normal blood donors serum hepatitis C virus RNA detection at the same time, serum anti-HCV detection. The results show that the method is sensitive and specific, and can be applied to the early clinical diagnosis of hepatitis C and blood donor screening.