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应用种子储藏蛋白电泳方法 ,对来源于CIMMYT的 2 0余份优质春小麦进行了醇溶蛋白位点Gli - 1和谷蛋白位点Glu - 1的遗传变异分析。结果表明 ,供试材料中Gli- 1位点存在较大的遗传多样性 ,其中Gli-B1l的频率较低 ,说明 1RS/1BL易位系较少 ,Gli- 1B位点亚基缺位也具有一定的比例 ,可能该位点对小麦品质没有负效应。在Glu - 1位点的等位基因的变异上 ,亚基 2 、17+ 18和 5 + 10所占的比例较高 ,故亚基的综合评分高。因此 ,可以在四川小麦育种中利用这些材料在种子醇溶蛋白组成上的变异 ,以丰富四川小麦的遗传多样性 ,在育种中注重改良1RS/1BL易位系品种的品质 ,同时转入优良的高分子谷蛋白亚基 ,进一步提高四川小麦中优质亚基的频率。另外 ,还对四川小麦育种利用国外优质小麦种质资源的策略进行了讨论
The genetic variations of Gli - 1 and Glu - 1 of gliadin loci in more than 20 high quality spring wheat cultivars derived from CIMMYT were analyzed using seed storage protein electrophoresis. The results showed that there was a large genetic diversity in the Gli-1 locus of the tested materials, of which the frequency of Gli-B1l was relatively low, indicating that the 1RS / 1BL translocation line was few and the absence of the Gli-1B locus subunit also had A certain percentage of this site may have no negative effect on wheat quality. The subunits 2, 17 + 18 and 5 + 10 account for a high proportion of alleles at the Glu - 1 locus, so the subgroups have high comprehensive scores. Therefore, the variation of seed gliadin composition in Sichuan wheat breeding could be used to enrich the genetic diversity of Sichuan wheat, and the quality of 1RS / 1BL translocation line was improved in breeding, High molecular glutenin subunit, to further improve the frequency of high quality subunits in Sichuan wheat. In addition, the strategy of using foreign high-quality wheat germplasm resources in Sichuan wheat breeding was also discussed