论文部分内容阅读
目的 :为了探讨用于胰岛移植的海藻酸钠 聚赖氨酸 海藻酸钠 (APA)生物膜的免疫隔离效果。方法 :采用高压静电成囊装置 ,制备APA微胶囊和微囊化胰岛 ,微囊直径为 0 2 5～ 0 5 5mm ;取空微囊 ,利用恒温振荡仪振荡后测定其破损率 ;将空微囊移植至小鼠腹腔 ,分别于不同时间由腹腔中灌洗出微胶囊 ,记数并观察其形态 ;取一定量空微囊分别与IgG、BSA和胰蛋白酶孵育 ,测量其浓度变化确定APA微胶囊的通透性。结果 :采用高压静电成囊技术制成的APA微胶囊呈球形 ,大小均匀、表面光滑 ,具有较好的生物相容性 ;粒径为 0 2 5～ 0 35mm的微胶囊其牢固度大于粒径为 0 45～ 0 5 5mm的微胶囊 ;葡萄糖、胰岛素等小分子物质能够自由通过微囊膜 ,胰蛋白酶也可通过 ,但速度较慢 ;大分子物质牛血清白蛋白和免疫球蛋白则不能透入APA微囊。结论 :采用高压静电成囊技术可制备高质量粒径为 0 2 5～ 0 35mm的微胶囊 ;这是具有良好免疫隔离性能的APA微胶囊。
Objective: To investigate the immunological isolation of sodium alginate polylysine alginate (APA) biofilm for islet transplantation. Methods: APA microcapsules and microencapsulated islets were prepared by using high voltage electrostatic encapsulation device. The diameter of the microcapsules was 0 25 ~ 0 5 5 mm. The microcapsules were taken out, and the damaged microcapsules were determined by shaking with a constant temperature oscillator. The cells were transplanted into the peritoneal cavity of mice. The microcapsules were perfused from the peritoneal cavity at different times and counted. Morphology was observed. A certain amount of empty microcapsules were incubated with IgG, BSA and trypsin, respectively. Capsule permeability. Results: The APA microcapsules made by high voltage electrostatic encapsulation technology were spherical, uniform in size and smooth in surface, with good biocompatibility. The microcapsules with particle size of 0 2 5 ~ 0 35mm had higher firmness 0 45 ~ 0 5 5mm microcapsules; glucose, insulin and other small molecules can freely through the capsule, trypsin can pass, but slower; macromolecular substances bovine serum albumin and immunoglobulin can not be through Into APA microcapsules. CONCLUSION: High-pressure electrostatic microcapsule can be used to prepare microcapsules with high quality particle size of 0 2 5 ~ 0 35mm; this is APA microcapsules with good immunosuppressive properties.