目前,有关多种水溶性维生素的高效液相色谱分离大都采用梯度洗脱或离子对色谱法。前者不仅操作不便,且重复性较差,会给定量分析带来困难;后者虽能改善分离,但离子对试剂价格昂贵,同时对柱填料有一定的腐蚀性。鉴于以上问题,我们用Lichrosorb RP-18柱,在甲醇-水体系中分别加入 Currently, most of the water-soluble vitamins HPLC separation using gradient elution or ion-pair chromatography. The former is not only inconvenient to operate, but also has poor repeatability, which brings difficulties to quantitative analysis. Although the latter can improve the separation, the ion-pairing reagent is expensive and has a certain corrosiveness to the column packing. In view of the above problems, we use Lichrosorb RP-18 column, respectively, methanol-water system were added