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为研究黄芪多糖(APS)对黄曲霉毒素B1(AFB1)诱发雏鸡肝损伤的防治作用,120只1日龄雏鸡随机分为空白对照组(A)、阳性对照组(B)和APS低、中、高剂量组保护组(C~E组)。B~E组分别在饲料中添加AFB12.8 mg/kg·7 d,C~E组在给予AFB_1的同时,分别在饮水中添加浓度为100 mg/m L、500 mg/m L、1 000 mg/m L的APS,连续给药21 d。各组分别在投药后的7、14、21、28 d取肝脏称重,计算肝指数,心脏采血,检测血清中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)的活性,白蛋白(ALB)、总蛋白(TP)和总胆红素(TBIL)的含量,并在7 d时制作肝脏病理切片。结果显示,连续给药7 d,500、1 000 mg/m L APS组有效拮抗AFB_1引起的脂肪变性,肝脏组织结构得到显著恢复,肝指数显著下降。肝功能生化指标ALT、AST、ALP活性,TBIL、ALB、TP含量与空白对照组无显著差异(P>0.05)。表明500 mg/m L或1 000 mg/m L APS可拮抗AFB1引起的雏鸡肝损伤,并有较好的防治作用。
In order to study the preventive and therapeutic effects of Astragalus polysaccharide (APS) on aflatoxin B1 (AFB1) -induced hepatotoxicity in chickens, 120 chickens of 1 day old were randomly divided into blank control group (A), positive control group (B) , High dose group protection group (C ~ E group). B and E groups were fed AFB12.8 mg / kg and 7 d respectively. In groups C and E, AFB_1 was given to each group at the same time, with concentrations of 100 mg / m L, 500 mg / m L and 1 000 mg / m L of APS, continuous administration of 21 d. The rats in each group were weighed at 7, 14, 21 and 28 days after administration, liver index and blood samples were collected, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), albumin (ALB), total protein (TP) and total bilirubin (TBIL) were measured. Liver pathological sections were made at 7 days. The results showed that 7d, 500 and 1 000 mg / m L APS group were able to effectively antagonize the fatty degeneration induced by AFB_1, the liver tissue structure was significantly recovered, and the liver index decreased significantly. The biochemical indexes of liver function ALT, AST, ALP activity, TBIL, ALB, TP content and the blank control group no significant difference (P> 0.05). The results showed that APS at 500 mg / m L or 1 000 mg / m L could antagonize AFB1-induced liver injury in chickens and had a good preventive and therapeutic effect.