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采自我国青海、陕西等省地11个寄生小麦的孢囊线虫群体经形态学鉴定为禾谷孢囊线虫(Heterodera avenae)。用PCR技术扩增获得的rDNA-ITS片段长度约为1 060 bp。用HinfⅠ、TaqⅠ、HpaⅡ、HaeⅢ、PstⅠ、AluⅠ等6种限制性内切酶酶切ITS扩增产物;青海10群体YBT10A、HY65A、HY61B、ZHZ162B、HY5B、HHX8A、GH132A、HY92A、HY127B、DT142A的6个酶的RFLP图谱完全一致;陕西YL4A群体的HaeⅢ、HinfⅠ和HpaⅡ酶切结果与青海群体的上述3种酶切结果相同,但AluⅠ和PstⅠ的酶切结果比青海孢囊线虫群体都多1条1 060 bp片段,而YL4A群体TaqⅠ酶切结果较为复杂。对比已知Avenae组成员RFLP图谱,青海群体与北京房山H.avenae群体的RFLP图谱一致;而陕西YL4A的AluⅠ图谱与H.avenae法国群体一致,PstⅠ和TaqⅠ却与Avenae组成员已知酶切结果均不一致。河南3个禾谷孢囊线虫群体除TaqⅠ酶切结果比青海CCN群体多1条520 bp片段以外,其他5种酶的RFLP都一致,而与澳大利亚的禾谷孢囊线虫H.avenae群体的RFLP图谱一致。
The cyst nematode populations collected from 11 parasitic wheat in Qinghai and Shaanxi provinces of China were morphologically identified as Heterodera avenae. The rDNA-ITS fragment amplified by PCR was about 1 060 bp in length. ITS amplified products were digested with 6 restriction enzymes, HinfⅠ, TaqⅠ, HpaⅡ, HaeⅢ, PstⅠ, AluⅠ and other products. Qinghai 10 populations including YBT10A, HY65A, HY61B, ZHZ162B, HY5B, HHX8A, GH132A, HY92A, HY127B and DT142A The results of HaeⅢ, HinfⅠ and HpaⅡ digestion of YL4A in Shaanxi province were the same as those of the three strains in Qinghai. However, the digestion results of AluⅠ and PstⅠ were more than that of Qinghai cyst nematode 1 060 bp fragment, while YL4A Taq Ⅰ digestion results more complicated. Compared with the RFLP map of Avenae group, the RFLP maps of Qinghai and Beijing avenophagus H.avenae were the same. However, the Alu Ⅰ map of Shaanxi YL4A was consistent with that of H.avenae France. However, PstⅠand TaqⅠhad no difference with the Avenae group Are inconsistent. The three other cereal cyst nematode populations in Henan had the same RFLP except for the one 520 bp fragment in TaqⅠresidue more than the CCN population in Qinghai and the RFLP The same map.