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目的：研究同源盒ＨＯＸ基因的表达在ＡＴＲＡ诱导白血病细胞分化中的作用。方法：利用我们建立的一种高效、简单的ｃＤＮＡ克隆方法－ＨＯＸ家庭基因指纹图技术，克隆ＨＬ－６０细胞内的ＨＯＸｃＤＮＡ基因。采用半定量ＲＴ－ＰＣＲ技术初步探讨ＡＴＲＡ对所克隆的ＨＯＸ基因表达的影响。结果：筛选出一个在ＨＬ－６０细胞内有表达的同源盒基因一ＨＯＸＡＩ基因ｃＤＮＡ片段，初步研究证实，此基因的ｍＲＮＡ水平在ＡＴＲＡ的作用下被上调。结论：ＡＴＲＡ可诱导ＨＬ－６０细胞内ＨＯＸＡ１基因表达增强。提示在ＡＴＲＡ诱导细胞分化而治疗恶性肿瘤的分子机制中，包括上调ＨＯＸＡ１基因表达而致白血病细胞分化成熟。
Objective: To study the role of homeobox HOX gene expression in ATRA-induced leukemia cell differentiation. METHODS: The HOX cDNA gene in HL-60 cells was cloned using a highly efficient and simple cDNA cloning method, HOX family gene fingerprinting. The effect of ATRA on the expression of the cloned HOX gene was investigated by semiquantitative RT-PCR. RESULTS: A homologous box gene-HOXAI gene cDNA fragment expressed in HL-60 cells was screened. Preliminary studies confirmed that the mRNA level of this gene was upregulated by ATRA. CONCLUSION: ATRA can induce the enhancement of HOXA1 gene expression in HL-60 cells. It is suggested that the molecular mechanism of ATRA in inducing cell differentiation to treat malignant tumors includes up-regulation of HOXA1 gene expression and the differentiation and maturation of leukemia cells.