目的了解小鼠在去势前与去势后,铁对骨吸收影响的差异。方法建立高铁小鼠模型,将小鼠随机分为假手术组(SHAM组)、高铁去势组(F+OVX组)、去势组(OVX组)。铁剂干预两周后去势。在去势前(雌激素存在)与去势7周后(雌激素缺乏)检测小鼠体重,血清铁蛋白;反转录聚合酶链反应(RT-PCR)检测胫骨内抗酒石酸酸性磷酸酶(TRAP)、降钙素受体(CTR)、基质金属蛋白酶9(MMP9)、组织蛋白酶K(cathepsin K)、雌激素受体α(ERα)、雌激素受体β(ERβ)表达情况,股骨远端Micro-CT三维分析,提取各组小鼠股骨骨髓细胞进行破骨细胞培养,抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞分化情况。结果各组小鼠体重无明显差异。F+OVX组铁蛋白含量明显升高(P<0.05)。Micro-CT分析表明:雌激素存在时,正常组与铁干预组骨密度及相关指标无明显差异(P>0.05);雌激素缺乏时,与OVX组比较,F+OVX组骨量下降更为严重(P<0.05)。半定量PCR结果显示:雌激素存在时,两组TRAP、CTR、MMP9、cathepsin K基因表达无统计学差异;雌激素缺乏时,F+OVX组各基因表达水平较OVX组显著升高(P<0.05)。去势后ERβ表达下降(P<0.05),ERα无明显差异。TRAP染色显示去势前各组小鼠破骨细胞数无明显差异(P>0.05);而去势后,F+OVX组破骨细胞数明显高于OVX组(P<0.05)。结论未去势时(有雌激素),FAC对骨吸收影响甚微;去势后(无雌激素),FAC能显著增强破骨活性。 Objective To understand the effect of iron on bone resorption before and after castration in mice. Methods The model of high-speed rail mice was established. The mice were randomly divided into SHAM group, F-OVX group and OVX group. Iron intervention castration after two weeks. Body weight and serum ferritin were measured before ovariectomized (estrogen was present) and ovariectomized for 7 weeks (estrogen-deficient); tibial tartrate-resistant tartrate-resistant acid phosphatase was detected by reverse transcription-polymerase chain reaction TRAP, CTR, MMP9, cathepsin K, ERα, ERβ, and distal femur End Micro-CT three-dimensional analysis, each group of mice femur bone marrow cells were cultured for osteoclast culture, tartrate-resistant acid phosphatase (TRAP) staining observed osteoclast differentiation. Results There was no significant difference in body weight of mice in each group. F + OVX group ferritin content was significantly increased (P <0.05). Micro-CT analysis showed that there was no significant difference between the normal group and the iron-treated group (P> 0.05) in the presence of estrogen and the decrease of the bone mass in the F + OVX group compared with the OVX group Serious (P <0.05). Semiquantitative PCR results showed that there was no significant difference in the expression of TRAP, CTR, MMP9 and cathepsin K between the two groups in the presence of estrogen. In the absence of estrogen, the expression of each gene in F + OVX group was significantly higher than that in OVX group (P < 0.05). ERβ expression decreased after castration (P <0.05), ERα no significant difference. TRAP staining showed that the number of osteoclasts in each group had no significant difference (P> 0.05) before castration, while the number of osteoclasts in F + OVX group was significantly higher than that in OVX group (P <0.05). Conclusions FAC has little effect on bone resorption without castration (with estrogen). After castration (without estrogen), FAC can significantly enhance osteoclast activity.