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目的 :探讨抗纤复方药物血清对肝星形细胞 (HSC)LI90细胞 (HSC LI90 )Ⅰ型、Ⅳ型前胶原和基质金属蛋白酶 (MMPs)及其组织抑制因子 (TIMP 1)基因表达的影响。方法 :以 0 5、2 0、4 0 g/kg等不同剂量抗纤复方灌胃大鼠 ,制备药物血清 ,作用于HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测Ⅰ型、Ⅳ型前胶原、基质金属蛋白酶 2 (MMP 2 )及膜型基质金属蛋白酶 (MT1 MMP)及其组织抑制因子(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。结果 :(1)抗纤复方不同浓度药物血清能抑制HSC LI90细胞Ⅰ型、Ⅳ型前胶原及其组织抑制因子TIMP 1的基因表达 (P <0 0 5或P <0 0 1) ;(2 )能增加膜型基质金属蛋白酶基因表达 (P <0 0 1) ;(3)对基质金属蛋白酶 2基因表达及活性无影响 (P >0 0 5 )。结论 :(1)抗纤复方具有抗肝纤维化作用 ;(2 )抗纤复方抑制HSC LI90细胞TIMP 1基因表达水平 ,促进胶原降解 ,可能是抗肝纤维化的作用机制之一。
Objective: To investigate the effect of anti-fibrosis compound serum on the expression of type I and type IV procollagen, matrix metalloproteinases (MMPs) and tissue inhibitor of transcription factor (TIMP 1) gene in hepatic stellate cell (HSC) LI90 cells (HSC LI90). METHODS: Rats were given intragastric administration of various doses of anti-fibrosis such as 0 5, 20, 40 g/kg to prepare drug serum and act on HSC LI90 cells for 48 hours. Northern blot hybridization was used to detect type I and type IV. The expressions of procollagen, matrix metalloproteinase 2 (MMP 2), membrane type matrix metalloproteinase (MT1 MMP) and tissue inhibitor of transcription factor (TIMP 1) gene were detected. The activity of matrix metalloproteinase 2 was detected by enzyme image method. Results: (1) Anti-fibrosis prescriptions with different concentrations of drug serum can inhibit gene expression of type I and type IV procollagen and tissue inhibitor of TIMP 1 in HSC LI90 cells (P <0 05 or P <0 01); (2 ) Increased expression of membrane-type matrix metalloproteinase gene (P <0 01); (3) No effect on expression and activity of matrix metalloproteinase 2 (P > 0 05). Conclusion: (1) Anti-fibrosis compound has anti-hepatic fibrosis effect; (2) Anti-fibrosis compound inhibits expression of TIMP 1 gene in HSC LI90 cells and promotes collagen degradation, which may be one of the mechanisms of anti-fibrosis.