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目的:以内毒素休克拟感染性休克,探讨补气活血解毒法在防治内毒素休克脑损伤中的保护作用与相关机制。方法::96只SD大鼠随机分为假手术组(生理盐水20 ml·kg~(-1))、模型组(脂多糖8 mg·kg~(-1))、加味生脉饮注射液组(脂多糖8mg·kg~(-1)加味生脉饮注射液10ml·kg~(-1))、地塞米松组(脂多糖8 mg·kg~(-1)+地塞米松5 mg·kg~(-1))等4组,每组再分为1,2,3,6 h 4个观测时点。各动物手术分离颈总动脉并与生物机能系统连接,监测平均动脉压(MAP),测定脑组织含水量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性等指标,观察脑组织结构的变化。结果:模型组动物在注射脂多糖后MAP进行性下降,脑含水量百分数明显增加至(82.69±0.28)%(P<0.05),MDA含量明显增加至(9.02±1.19)nmol·mg~(-1)(P<0.05),SOD活性明显下降至(37.15±2.92)U·mg~(-1)(P<0.05),同时观察到脑微血管水肿,各治疗组上述病理改变明显改善。结论:生脉饮加味方有较好的抗休克、保护脑组织的作用,补气活血解毒治法可作为感染性休克中后期的中医治法。
OBJECTIVE: To study the protective effect and the related mechanism of “qi-activating and blood-activating detoxification” method in the prevention and treatment of endotoxic shock brain injury. Methods: Ninety-six SD rats were randomly divided into sham operation group (20 ml · kg -1 saline), model group (8 mg · kg -1 lipopolysaccharide), plus Shengmai Yin injection The rats in the dexamethasone group (8 mg · kg -1, LPS plus 10 mg · kg -1) and dexamethasone 5 mg · Kg ~ (-1)) and other 4 groups, each group is divided into 1,2,3,6 h 4 observation time points. The animals were surgically isolated from the common carotid artery and connected with a bio-functional system to monitor mean arterial pressure (MAP), brain water content, malondialdehyde (MDA) content and superoxide dismutase (SOD) Changes in organizational structure. Results: The MAP decreased and the content of brain water increased to (82.69 ± 0.28)% and (9.02 ± 1.19) nmol · mg ~ (-) in model group after injection of lipopolysaccharide (P <0.05) 1) (P <0.05). The activity of SOD decreased to (37.15 ± 2.92) U · mg ~ (-1) (P <0.05). Cerebral microvascular edema was also observed. The above pathological changes were significantly improved in all treatment groups. Conclusion: Shengmaiyin flavored prescription has good anti-shock, the role of protecting brain tissue, Buqi Huoxue detoxification therapy can be used as the Chinese medicine treatment of septic shock in the late.