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目的探讨基因甲基化在雄激素非依赖性前列腺癌(androgen-independent prostate cancer,AIPC)转化过程中可能的作用。方法采用重亚硫酸盐测序PCR(bisulfite genomic sequencing PCR,BSP)联合TA克隆测序检测雄激素依赖性前列腺癌(androgen-dependent prostate cancer,ADPC)细胞株LNCaP和雄激素非依赖性前列腺癌细胞株LNCaP-AI(androgen independent)中生长因子受体结合蛋白10(growth factor receptor-bound protein 10,GRB10)和B细胞性淋巴瘤/白血病-2基因(B-cell lymphoma/leukaemia-2 gene,BCL2)的甲基化状态。结果 GRB10基因在LNCaP-AI细胞和LNCaP细胞中的甲基化率分别为9.6%、7.4%;BCL2基因在LNCaP-AI细胞和LNCaP细胞中的甲基化率分别为14.7%、25.3%,这两个基因在LNCaP细胞和LNCaP-AI细胞的甲基化率差异无统计学意义(P>0.05)。结论 GRB10和BCL2基因的异常表达与基因甲基化无明显相关,而二者是否参与到前列腺癌激素非依赖性到转化过程以及具体机制有待进一步研究。
Objective To investigate the possible role of gene methylation in the process of androgen-independent prostate cancer (AIPC) transformation. Methods The expression of LNCaP in androgen-dependent prostate cancer (ADPC) cell line LNCaP and androgen-independent prostate cancer cell line LNCaP-LNCaP were detected by bisulfite genomic sequencing PCR (BSP) A (androgen independent) A gene of growth factor receptor-bound protein 10 (GRB10) and B-cell lymphoma / leukemia-2 gene (BCL2) Base state. Results The methylation rates of GRB10 gene in LNCaP-AI cells and LNCaP cells were 9.6% and 7.4%, respectively. The methylation rates of BCL2 gene in LNCaP-AI cells and LNCaP cells were 14.7% and 25.3%, respectively The methylation rates of two genes in LNCaP cells and LNCaP-AI cells were not statistically different (P> 0.05). Conclusion Abnormal expression of GRB10 and BCL2 genes is not related to gene methylation. Whether they are involved in the hormone-independent transition to prostate cancer remains to be further studied.