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AIM: To observe the possible effects of transforming growth factor (TGF) β1, interleukin (IL)-6, tumor-necrosis factor (TNF) α and IL-10 on experimental rat hepatic fibrosis.METHODS: One hundred SD rats were divided randomly into the three groups. Control group received intraperitoneal injection of saline (2 ml.kg-1), twice a week. Fibrogenesis group was injected intraperitoneally with 50% carbon 20 minutes before CCl4 administration from the third week.At the fifth, seventh, and ninth weeks, 7 to 10 rats in each group were sacrificed to collect serum. Levels of TGF-β1,TNF-α, IL-6 and IL-10 were determined by enzyme-linked immunosorbent assay (ELISA). The liver tissues were taken for routine histological examination.RESULTS: Hepatic fibrosis was developed with the injection of CCl4. Values of the circulating TGFβ1, TNFα, IL-6 and IL-respectively. Their levels in the CCl4-intoxication group were gradual increase was observed in the levels of TGFβ1, TNFαand IL-6 during hepatic fibrogenesis. These changes were partially reversed by simultaneous administration of IL-10. The histological parameters, characterized by CCl4-intoxification,also seemed to be improved with IL-10 treatment, the collagen production was reduced at the ninth week and the histological activity index was decreased from 7.9±1.2 to 4.7±0.9.CONCLUSION: TGFβ1, TNFα and IL-6 may play important roles during CCl4-induced hepatic fibrogenesis, and IL-10 may counterbalance their effects.