论文部分内容阅读
从1800m到3400m五个海拔连续取样,用RAPD分子标记研究了卧龙自然保护区中国沙棘种群的遗传结构和遗传变异.用11条寡核苷酸引物,扩增得到151个重复性好的位点,其中143个多态位点,多态率达94.7%.在5个沙棘种群中,总遗传多样性值(HT)为0.289,B种群内的遗传多样性值为0.315,这完全符合沙棘这种多年生、远交的木本植物高遗传变异的特性.5个种群内遗传多样性随海拔升高呈低—高—低变异趋势,在2200m海拔处的B种群遗传多样性达最大值0.315,3400m海拔处的E种群则表现最小,仅0.098.5个种群间的遗传分化值GST=0.406,也即是说有40.6%的遗传变异存在于种群间,59.4%存在种群内.1800m海拔处的A种群与其他种群的明显分离是造成种群间遗传分化大的原因.UPGMA聚类图和PCoA散点图分别进一步确证了5个种群间关系和所有个体间的关系.最后,经过Mantel检测,遗传距离与海拔表现了明显的相关性(r=0.646,P=0.011).
From 1800m to 3400m, the five consecutive altitudes were sampled and the genetic structure and genetic variation of Chinese seabuckthorn population in Wolong Nature Reserve were studied using RAPD markers. With 11 oligonucleotide primers, 151 repeats were amplified , Of which 143 polymorphic loci had a polymorphism rate of 94.7%. Among 5 seabuckthorn populations, total genetic diversity (HT) was 0.289 and genetic diversity within B was 0.315, which was in full accord with Perennial and outcrossing.The genetic diversity of five populations showed the trend of low-high-low variation with altitude, the genetic diversity of population B at the altitude of 2200m was up to 0.315, The E population at 3400 m above sea level was the smallest, with genetic differentiation GST = 0.406 only for 0.098.5 population, which means that 40.6% of the genetic variation existed among the populations and 59.4% within the population. The obvious segregation of A population from other populations was the major reason for the genetic differentiation among the populations.The UPGMA cluster and PCoA scatter plots further confirmed the relationship between five populations and all individuals respectively.Finally, Distance and altitude show a clear correlation (R = 0.646, P = 0.011).