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目的建立高效液相色谱-串联质谱法同时测定果汁中展青霉素、乐果和多菌灵的方法。方法果汁采用Qu ECh ERS前处理方法,以C18色谱柱为分离柱,以水-乙腈为流动相进行梯度洗脱,用高效液相色谱-串联质谱(HPLCMS/MS),电喷雾电离(ESI),多反应监测(MRM)模式检测,外标法同时定量测定展青霉素、乐果和多菌灵。结果方法的线性范围展青霉素为5~500μg/L、乐果为0.1~10μg/L、多菌灵为0.1~10μg/L,线性相关系数在0.9991~0.9999之间,展青霉素检出限为5.0μg/L、乐果检出限为0.01μg/L、多菌灵检出限为0.01μg/L;本方法平均加标回收率82.2%~90.0%之间;相对标准偏差(RSD)均小于5.3%。结论该方法可应用于同时定量检测果汁中展青霉素、乐果和多菌灵,操作简单、快速、准确可靠。
Objective To establish a method for the simultaneous determination of patulin, dimethoate and carbendazim in fruit juice by high performance liquid chromatography-tandem mass spectrometry. Methods The juice was pretreated with Qu ECh ERS. The separation was performed on a C18 column with gradient elution with water-acetonitrile as mobile phase. High performance liquid chromatography-tandem mass spectrometry (HPLCMS / MS), electrospray ionization (ESI) , Multiple reaction monitoring (MRM) mode detection, external standard method for the simultaneous determination of patulin, dimethoate and carbendazim. Results The linear range of the method was 5 ~ 500μg / L for patulin, 0.1 ~ 10μg / L for dimethoate and 0.1 ~ 10μg / L for carbendazim. The linear correlation coefficient was between 0.9991 and 0.9999. The detection limit of patulin was 5.0 μg / L, the limit of detection of dimethoate was 0.01 μg / L and the limit of detection of carbendazim was 0.01 μg / L. The average recoveries of this method were between 82.2% and 90.0%; the relative standard deviations (RSDs) 5.3%. Conclusion The method can be applied to the simultaneous quantitative determination of patulin, dimethoate and carbendazim in fruit juice. The method is simple, rapid, accurate and reliable.