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目的探讨小剂量阿司匹林对糖尿病大鼠心脏的保护作用及机制。方法雄性SD大鼠尾静脉注射链脲佐菌素30mg/kg,造成1型糖尿病动物模型后,随机分为糖尿病模型组和阿司匹林组各8只,另选8只健康雄性大鼠为对照组。阿司匹林组大鼠灌服阿司匹林9mg/kg+蒸馏水,对照组和糖尿病模型组大鼠灌服同体积的蒸馏水,12周后检测各组大鼠空腹血糖、血清肌酸激酶(creatine kinase,CK)和乳酸脱氢酶(lactate dehydrogenase,LDH)水平,HE染色法检查心肌组织病理显微结构,免疫组织化学法检测Caspase-3,Bax和Bcl-2的表达情况。结果糖尿病模型组和阿司匹林组大鼠空腹血糖、CK和LDH水平较对照组明显升高(P<0.05),阿司匹林组空腹血糖较糖尿病模型组降低(P<0.05);阿司匹林组CK和LDH水平较糖尿病模型组降低,但差异均无统计学意义(P>0.05);糖尿病模型组大鼠心脏显微结构基本正常,但可见局部心肌纤维排列紊乱等病理改变,小剂量阿司匹林治疗12周后,心肌上述病理改变明显减轻;糖尿病模型组Caspase-3和Bax表达均强于对照组(P<0.05),Bcl-2表达弱于对照组(P<0.05),Bcl-2/Bax比值低于对照组(P<0.05);阿司匹林组Caspase-3和Bax表达均弱于糖尿病模型组(P<0.05),Bcl-2表达明显强于糖尿病模型组(P<0.05),Bcl-2/Bax比值明显高于糖尿病模型组(P<0.05)。结论小剂量阿司匹林可能通过调节细胞凋亡相关蛋白表达对糖尿病大鼠心脏起保护作用。
Objective To investigate the protective effect of low-dose aspirin on the heart of diabetic rats and its mechanism. Methods Male SD rats were injected streptozotocin (30mg / kg) via the tail vein to induce type 1 diabetic animal models and then randomly divided into diabetic model group and aspirin group (n = 8). Eight healthy male rats were selected as the control group. The rats in the aspirin group were given aspirin 9 mg / kg and distilled water. The rats in the control group and diabetic model group were fed with the same volume of distilled water. After 12 weeks, the fasting blood glucose, serum creatine kinase (CK) and lactate The levels of LDH, HE staining were used to detect the pathological changes of myocardial tissue. The expressions of Caspase-3, Bax and Bcl-2 were detected by immunohistochemistry. Results The levels of fasting blood glucose, CK and LDH in diabetic model group and aspirin group were significantly higher than those in control group (P <0.05). The fasting blood glucose in aspirin group was lower than that in diabetic model group (P <0.05). The levels of CK and LDH in aspirin group were significantly higher than those in control group Diabetic model group decreased, but the difference was not statistically significant (P> 0.05); diabetic model group rats cardiac normal microstructure, but we can see local myocardial fiber disorder and other pathological changes, low-dose aspirin after 12 weeks of treatment, myocardial The expression of Caspase-3 and Bax in diabetic model group was significantly higher than that in control group (P <0.05), Bcl-2 expression was weaker than that in control group (P <0.05), and the ratio of Bcl-2 / Bax was lower than that in control group (P <0.05). The expressions of Caspase-3 and Bax in aspirin group were weaker than those in diabetic model group (P <0.05), while the expression of Bcl-2 was significantly stronger in diabetic aspirin group than that in diabetic model group In diabetic model group (P <0.05). Conclusion Low-dose aspirin may protect the heart of diabetic rats by regulating the expression of apoptosis-related proteins.