论文部分内容阅读
目的建立辅助降血糖类保健食品中18种非法添加物的高效液相色谱-质谱(HPLC-MS)定性定量检测方法。方法采用HPLC-MS检测,试样以甲醇提取后,经Waters Atlantis T3 C18(2.1×150 mm,5μm)色谱柱分离,以0.01 mol/L乙酸铵(含0.1%甲酸)和甲醇为流动相梯度洗脱,采用ESI+、MRM模式检测。结果 18种非法添加物在相应浓度范围内线性关系良好,相关系数均在0.999以上,检出限均在0.025~0.05μg/g范围内,仪器精密度RSD为0.3%~3.5%,回收率为66.4%~116.9%。结论该方法快速、准确、灵敏度高,可作为辅助降血糖类保健食品中非法添加化学降糖药的快速筛查和测定方法。
Objective To establish a qualitative and quantitative HPLC-MS method for the determination of 18 illegal additives in health foods that help to lower blood sugar. Methods The HPLC-MS method was used. The samples were extracted with methanol and separated on a Waters Atlantis T3 C18 (2.1 × 150 mm, 5 μm) column. The mobile phase consisted of 0.01 mol / L ammonium acetate (containing 0.1% formic acid) and methanol Elution, using ESI +, MRM mode detection. Results 18 kinds of illegal additives had good linearity in the corresponding concentration range with the correlation coefficients above 0.999. The detection limits were in the range of 0.025 ~ 0.05 μg / g. The RSD of the instrument was 0.3% ~ 3.5%. The recovery rate was 66.4% ~ 116.9%. Conclusion The method is rapid, accurate and sensitive and can be used as a rapid screening and determination method for illegally adding chemical hypoglycemic agents in health foods that help hypoglycemic.