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本文设计合成了两种光敏的双亲性分子,分别具有单齿和三齿的结构,这两种双亲性分子可自组装成球形和楔形超分子纳米粒。超分子外部带有氨基的PEG片段可以通过静电作用与核酸结合。本文对超分子输送寡聚核苷酸进入细胞的效果进行了评价。结果表明,具有三齿结构的分子与核酸形成的复合物通过内吞作用可以有效地将核酸输送进入细胞,而单齿结构的复合物却几乎不具有此能力。给予光照后,三齿的超分子发生醌甲基化重排而解离,从而释放核酸。
In this paper, we designed and synthesized two photosensitive amphiphilic molecules with monodentate and tridentate structures, respectively. These amphipathic molecules can be self-assembled into spherical and wedge-shaped supramolecular nanoparticles. The PEG fragment with amino groups on the outside of the supramolecule can bind to the nucleic acid by electrostatic interaction. In this paper, the effect of supramolecular delivery of oligonucleotides into cells was evaluated. The results show that the complexes formed by the tridentate molecules and the nucleic acids can efficiently deliver the nucleic acids into the cells by endocytosis, whereas the monodentate complexes hardly have this capability. Given light, the tridentate supramolecules undergo quinone methylation rearrangement and dissociate, releasing the nucleic acid.