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目的 探讨原发性肾病综合征 ( PNS)患者发病期及缓解期的细胞免疫状态。方法 分别采用单克隆抗体致敏红细胞检测法、白介素 2 ( IL - 2 )生物活性检测法和受体的放射性配体结合法 ,对 39例 PNS患者及 2 5例正常对照的外周血淋巴细胞亚群、IL - 2活性及高亲和力白介素 2受体 ( IL - 2 R)的表达进行检测。结果 PNS组2 2例发病期患者的 T淋巴细胞 ( CD3 )、辅助淋巴细胞 ( CD4)、抑制淋巴细胞 ( CD8)以及 IL - 2、IL - 2 R均显著低于对照组 ( P<0 .0 5 ) ;17例缓解期患者的 CD3 、CD4、IL - 2、IL - 2 R高于发病期组 ,但低于对照组 ,且与后两组的上述各项指标均有显著性差异 ( P<0 .0 5 )。结论 PNS患者发病期细胞免疫功能低下 ,缓解期有所恢复 ,但仍未达正常水平。缓解期细胞免疫功能低下可能是 PNS易于复发的原因之一。
Objective To investigate the cellular immune status during the onset and remission of patients with primary nephrotic syndrome (PNS). Methods Monoclonal antibody sensitized erythrocyte assay, interleukin 2 (IL - 2) bioassay and radioligand binding assay were used to detect the expression of interleukin - 2 (IL - 2) in peripheral blood lymphocytes of 39 patients with PNS and 25 normal controls The activity of IL - 2 and the expression of interleukin - 2 receptor (IL - 2R) with high affinity were detected. Results The T lymphocyte (CD3), Helper lymphocyte (CD4), CD8, IL - 2 and IL - 2 in 22 patients with PNS were significantly lower than those in the control group (P <0. 0.05). The levels of CD3, CD4, IL - 2 and IL - 2R in 17 remission patients were higher than those in the onset stage, but lower than those in the control group (P <0.05). The above indexes were significantly different from those in the latter two groups P <0. 05). Conclusions The cellular immune function of patients with PNS during the onset is low, and the remission period has been restored, but still not reached the normal level. Remission cellular immune dysfunction may be one of the reasons PNS easy to relapse.