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目的：探讨ａｐｏＥ 基因摧毁鼠血浆极低密度脂蛋白和中间密度脂蛋白组分（ａｐｏＥｋｏ － ＶＬＤＬ／ＩＤＬ） 致动脉硬化作用。方法：检测ａｐｏＥｋｏ － ＶＬＤＬ／ＩＤＬ 的理化性质，包括脂含量，电泳迁移率和载脂蛋白组成，并观察了该组分与Ｊ７７４巨噬细胞的相互作用。结果：ａｐｏＥｋｏ － ＶＬＤＬ／ＩＤＬ 可被Ｊ７７４ 巨噬细胞摄取，导致细胞胆固醇酯（ＣＥ） 大量蓄积（ Ｐ＜０－０１） 。结论：ａｐｏＥ 缺失鼠血浆非修饰ａｐｏＥｋｏ － ＶＬＤＬ／ＩＤＬ 组分以不依赖ａｐｏＥ 方式转Ｊ７７４ 巨噬细胞为泡沫细胞，参与动脉粥样硬化形成
AIM: To investigate the effect of apoE gene on aortic arteriosclerosis induced by apoEko - VLDL / IDL in plasma of rats. Methods: The physico - chemical properties of apoEko - VLDL / IDL including lipid content, electrophoretic mobility and apolipoprotein composition were determined and the interaction between this component and J774 macrophages was observed. Results: apoEko - VLDL / IDL was uptake by J774 macrophages, leading to a large accumulation of cellular cholesterol esters (CE) (P <0-01). CONCLUSION: ApoE-deficient murine plasma unmodified apoEko - VLDL / IDL components are involved in the formation of atherosclerosis in apoE-directed transfection of J774 macrophages into foam cells