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目的:探讨pre-miR-146a基因表达及其rs2910164位点多态性与胆管癌的关系。方法:分别用基因直接测序与定量PCR方法检测70例胆管癌患者的胆管癌组织(胆管癌组)及39例胆管非肿瘤性疾病患者的胆管组织(对照组)中pre-miR-146a基因rs2910164位点单核苷酸多态性与pre-miR-146a表达,分析不同基因型与pre-miR-146a表达量、胆管癌临床病理及其预后的关系。结果:胆管癌组的pre-miR-146a基因型分布与对照组有明显差异,前者表现为GG和GC基因型比例明显高于CC基因型,且G等位基因频率明显高于C等位基因(均P<0.05);对照组GG和GC基因型人群的pre-miR-146a表达量较CC基因型低,但差异无统计学意义(P>0.05),胆管癌组织pre-miR-146a表达量明显低于对照组胆管组织(P<0.05);Logistic多元回归分析显示GG和GC基因型可能是胆管癌的危险因素(P=0.052),分层分析显示GG和GC基因型与胆管癌患者的临床分期和淋巴结转移有关(均P<0.05);生存分析显示GG和GC基因型胆管癌患者的生存率低于CC基因型胆管癌患者,但差异无统计学意义(P=0.178)。结论:pre-miR-146a基因rs2910164位点G等位基因频率升高可能是导致pre-miR-146a基因表达降低以及胆管癌发生发展的危险因素。
Objective: To investigate the relationship between pre-miR-146a gene expression and rs2910164 polymorphism and cholangiocarcinoma. Methods: The gene expression profiles of pre-miR-146a rs2910164 in 70 cases of cholangiocarcinoma (cholangiocarcinoma) and 39 cases of biliary non-neoplastic disease (control group) were detected by direct sequencing and quantitative polymerase chain reaction Site single nucleotide polymorphisms and pre-miR-146a expression, analyze the relationship between different genotypes and pre-miR-146a expression, clinicopathological features and prognosis of cholangiocarcinoma. Results: The distribution of pre-miR-146a genotype in cholangiocarcinoma group was significantly different from that in control group. The former showed a significantly higher proportion of GG and GC genotypes than CC genotype, and the G allele frequency was significantly higher than that of C allele (All P <0.05). The expression of pre-miR-146a in control group was lower than that in CC genotype in GG and GC genotypes, but the difference was not statistically significant (P> 0.05). The expression of pre-miR-146a in cholangiocarcinoma Logistic multiple regression analysis showed that GG and GC genotypes may be risk factors for cholangiocarcinoma (P = 0.052). Hierarchical analysis showed that GG and GC genotypes were significantly different from those in cholangiocarcinoma patients (P <0.05). Survival analysis showed that the survival rate of patients with GG and GC genotype cholangiocarcinoma was lower than that of CC genotype cholangiocarcinoma, but the difference was not statistically significant (P = 0.178). Conclusion: The increased frequency of G allele at rs2910164 in pre-miR-146a gene may be the risk factor for the decrease of pre-miR-146a gene expression and the occurrence and development of cholangiocarcinoma.