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目的:研究兔后肢动脉生成过程中诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)表达特征。方法:将兔一侧股动脉结扎,另一侧设为对照组。1周后动物被处死。应用免疫荧光组织化学技术检测侧支血管中iNOS及eNOS的表达。用Leica激光共聚焦显微镜观察并拍照,图片用silicon graphicsoctane进行处理。结果:在正常小动脉血管中iNOS的表达很低,在生长的侧支血管iNOS的表达显著上调,是正常小动脉血管的2.8倍。其表达在血管壁的各层可见。正常小动脉血管eNOS的表达呈现一定基础水平,在生长的侧支血管中eNOS的表达呈强阳性,集中在内皮细胞,是正常小动脉血管的2.2倍。结论:侧支血管发育过程中iNOS的表达上调,上调的iNOS和eNOS可能通过生成NO,调节内皮细胞的增殖、移动及炎症的形成,从而对侧支血管的生长发挥重要作用。
AIM: To investigate the expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in rabbit hindlimb arteries. Methods: Rabbit femoral artery ligation, the other side as the control group. Animals were sacrificed after 1 week. Immunofluorescence histochemistry was used to detect the expression of iNOS and eNOS in collateral vessels. Observed with a Leica laser confocal microscope and photographed, the picture was treated with silicon graphicsoctane. RESULTS: The expression of iNOS in normal arterioles was very low. The expression of iNOS in collateral vessels was significantly up-regulated, which was 2.8-fold that of normal arterioles. Its expression is visible on all layers of the vessel wall. The expression of eNOS in normal arterioles showed a certain basic level. The expression of eNOS in collateral vessels was strongly positive and concentrated in endothelial cells, which was 2.2 times that of normal arterioles. CONCLUSION: The expression of iNOS is up-regulated during the development of collateral vessels. Up-regulated iNOS and eNOS may play an important role in the growth of collateral vessels by regulating the formation of NO and regulating the proliferation, migration and inflammation of endothelial cells.