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目的探讨长期大蒜素干预下SD大鼠血清对食管癌EC109细胞增殖以及凋亡的影响。方法采用普通SD大鼠血清和长期大蒜素干预条件下SD大鼠血清培养食管癌EC109细胞。Ⅰ组:以普通灭活SD大鼠血清培养液培养食管癌EC109细胞;Ⅱ组:以大蒜素干预SD大鼠灭活血清培养液培养食管癌EC109细胞;应用流式细胞术测定细胞周期分布,并计算细胞S期分数和增殖指数;采用AnnexinⅤ-EGFP/PI双染法和亚倍体峰法应用流式细胞仪检测早期和中晚期凋亡率。结果Ⅱ组G2/M期及S期细胞比例明显低于Ⅰ组,而G0/G1期细胞比例明显升高;Ⅱ组S期分数及细胞增殖指数显著低于Ⅰ组(P<0.05),差异有统计学意义。Ⅱ组细胞凋亡率明显高于Ⅰ组,差异有统计学意义(P<0.05)。结论长期大蒜素干预下SD大鼠血清对食管癌EC109细胞的增殖活性具有明显的抑制作用并能促进食管癌EC109细胞的凋亡。
Objective To investigate the effects of long-term allicin serum on the proliferation and apoptosis of esophageal carcinoma EC109 cells. Methods The esophageal carcinoma EC109 cells were cultured in SD rat serum with the intervention of normal SD rat serum and long-term allicin. GroupⅠ: EC109 cells were cultured in normal inactivated SD rat serum culture medium; GroupⅡ: Allogeneic SD rats inactivated serum culture medium was used to culture EC109 esophageal cancer cells; Cell cycle distribution was analyzed by flow cytometry, The S phase score and proliferation index of the cells were calculated. The apoptosis rate of early and middle stages was detected by AnnexinⅤ-EGFP / PI double staining and the double-peak method. Results The proportion of cells in G2 / M phase and S phase in group Ⅱ was significantly lower than that in group Ⅰ, while the proportion of cells in G0 / G1 phase was significantly increased. The S phase and cell proliferation index in group Ⅱ were significantly lower than those in group Ⅰ (P <0.05) There is statistical significance. The apoptosis rate of group Ⅱ was significantly higher than that of group Ⅰ, the difference was statistically significant (P <0.05). Conclusion The long-term allicin serum from SD rats can significantly inhibit the proliferation of esophageal carcinoma EC109 cells and promote the apoptosis of esophageal carcinoma EC109 cells.