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Spontaneous vesicles from the aqueous mixtures of dodecyl sulfonate betaine (DSB) and sodium bis (2-ethylhexyl) sulfosuccinate (AOT) at certain mixingratios have been demonstrated by using calorimetry, freeze-frac- ture transmission electronic microscopy (TEM), negative- staining TEM and dynamic light scattering (DLS) methods. The addition of NaCl will promote vesicle formation and the heat effect of monodisperse vesicle system is greatest. Meanwhile the mechanism was analyzed from the viewpoint of packing parameter f, molecular geometry structure and interaction.