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目的 通过检测大肠癌患者细胞因子水平了解其是否存在Th1与Th2细胞因子的不平衡及肿瘤免疫缺陷 ,了解细胞因子与临床病理的关系。方法 62例患者及 15例正常志愿者参与本研究 ,分别抽取外周晨血测定IL 2、IL 6、IL 10、IL 12、TNF及测定NK、CD3 +、CD4 +、CD8+。结果IL 2、IL 12水平在肿瘤组显著下降 ,IL 12与Dukes分期相关。IL 6、IL 10在肿瘤组中显著升高 (P <0 0 5 ) ,DukesD期患者升高更显著 ,IL 10还与肿瘤大小和病理分期有关。肿瘤组NK活性、CD4 +及CD4 +/CD8+比例降低。结论 大肠癌患者出现以Th1为主的细胞因子降低及Th2细胞因子的升高 ,肿瘤免疫缺陷。部分细胞因子代谢紊乱可能与大肠癌的不良预后有关。
Objective To detect the cytokine levels in patients with colorectal cancer to find out whether there is imbalance of Th1 and Th2 cytokines and tumor immune deficiency, to understand the relationship between cytokines and clinicopathological. Methods Sixty-two patients and 15 normal volunteers were enrolled in this study. Peripheral blood samples were collected for determination of IL 2, IL 6, IL 10, IL 12, TNF and NK, CD3 +, CD4 + and CD8 +. Results The levels of IL 2 and IL 12 were significantly decreased in the tumor group, while IL 12 was correlated with Dukes stage. IL-6 and IL-10 were significantly increased in the tumor group (P <0 05). The patients in DukesD stage were more significantly elevated. The level of IL-10 also correlated with tumor size and pathological stage. NK activity in the tumor group, CD4 + and CD4 + / CD8 ratio decreased. Conclusion Colorectal cancer patients with Th1-based cytokines and Th2 cytokines increased tumor immunodeficiency. Some disorders of cytokine metabolism may be related to the poor prognosis of colorectal cancer.