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筛选并利用优化的染色和透明实验方法,结合激光共聚焦扫描显微镜观察油茶成熟胚囊内部结构。结果表明,采用1%曙红染色1 h并用水杨酸甲酯透明5 d是观察油茶成熟胚囊较好的实验处理方案,胚囊内细胞轮廓清晰,对所需观察的目标有很好的表现力。基于该优化体系,构建了油茶胚囊的三维结构,为油茶及山茶属植物胚囊发育、受精机理等方面的研究提供了更加快速、高效的观察方法。
Screening and using optimized staining and transparent experimental methods, combined with laser confocal scanning microscope microscope observation of the internal structure of Camellia mature embryo sac. The results showed that 1% eosin staining for 1 h and methyl salicylate for 5 days were a good experimental method for observing the mature embryo sacs of Camellia oleifera. The outline of the cells in the embryo sac was clear and the target of observation was good Expressive. Based on this optimization system, the three-dimensional structure of Camellia oleifera embryo sac was constructed, which provided a more rapid and efficient observation method for the research on embryo sac development and fertilization mechanism of Camellia oleifera and Camellia.