论文部分内容阅读
AIM: To investigate the role of quercetin (Que) in the proliferation of cultured human skin microvascular endothelial cells (MVEC). METHODS: Cell count and [methyl-~3H]thymidine ([~3H]TdR) uptake assay were used to measure the effect of Que in the proliferation of cultured MVEC. Cytotoxicity of Que on MVEC was also evaluated by ~(51)Cr release assay. RESULTS: When MVEC were treated with Que, the proliferation was significantly inhibited in a time-course and dose-dependent manner. Que 5 μmol/L did not inhibit the proliferation of MVEC. When the concentration of Que increased to 20, 40, 80, and 160μmol/L, the cell numbers per well were decreased and the inhibition rate was 12.2％, 23.5％, 35.3％, and 54.1％ respectively with IC_(50) of 138 μmol/L. The inhibitory rate of [~3H]-TdR uptake was 18.7％, 34.4％, 48.9％, and 62.5％ respectively (IC_(50)=87.5 μmol/L). ~(51)Cr release assay showed that Que 160μmol/L incubated with MVEC from 1 to 16h had no clear cytotoxicity compared with control group. CONCL
AIM: To investigate the role of quercetin (Que) in the proliferation of cultured human skin microvascular endothelial cells (MVEC). METHODS: Cell count and [methyl- ~ 3H] thymidine ([~ 3H] TdR) uptake assay were used to measure The effect of Que in the proliferation of cultured MVEC. Cytotoxicity of Que on MVEC was also evaluated ~ (51) Cr release assay. RESULTS: When MVEC were treated with Que, the proliferation was significantly inhibited in a time-course and dose- When the concentration of Que increased to 20, 40, 80, and 160 μmol / L, the cell numbers per well were decreased and the inhibition rate was 12.2%, 23.5 %, 35.3%, and 54.1% respectively with IC 50 of 138 μmol / L. The inhibitory rate of [~ 3H] -TdR uptake was 18.7%, 34.4%, 48.9% and 62.5% respectively (IC 50) = 87.5 μmol / L). (51) Cr release assay showed that Que 160 μmol / L incubated with MVEC from 1 to 16 h had no clear cytotoxicity with control group. CONCL