论文部分内容阅读
研究了硫丹对草鱼肝脏Ⅰ相酶氨基比林-N-脱甲基酶(APND)和红霉素-N-脱甲基酶(ERND)、Ⅱ相酶谷胱甘肽-S-转移酶(GST)活性及DNA受损细胞彗星尾长(TL)和尾部DNA含量(%TDNA)的影响。试验共设置0.18、0.36和0.71μg.L-1 3个暴露浓度组和1个空白对照组,分别在试验24、72、120和168 h时取样测定各指标。结果表明,24 h时,0.36和0.71μg.L-1暴露组草鱼肝脏APND活性与对照组相比显著升高(P<0.05或P<0.01),72 h时受到显著抑制(P<0.01);120 h后各暴露组APND活性与对照组相比均表现为受到显著抑制(P<0.05或P<0.01)。ERND活性总体表现为受诱导。GST活性总体呈先受诱导后受抑制的变化趋势;0.36和0.71μg.L-1暴露组GST活性均在72 h时达到最高值,之后随暴露时间的延长缓慢降低,并在168 h时表现为受抑制;0.18μg.L-1暴露组在120 h时达到最大值,之后降低,168 h时GST活性与对照组水平相当。经硫丹暴露后,草鱼肝脏细胞DNA明显受损,TL与%TDNA均随硫丹浓度的升高或暴露时间的延长而增加,且相关显著。硫丹可影响草鱼肝脏Ⅰ相、Ⅱ相代谢酶活性,并对肝细胞DNA造成遗传损伤。
The effects of endosulfan on the phase Ⅰ enzyme aminopyrine-N-demethylase (APND) and erythromycin-N-demethylase (ERND), the phase Ⅱ enzyme glutathione S-transferase (GST) activity and DNA tail of damaged cells (TL) and tail DNA content (% TDNA). A total of 0.18,0.36 and 0.71μg.L-1 exposure concentration groups and one blank control group were set up, and samples were measured at 24, 72, 120 and 168 hours respectively. The results showed that at 24 h, the activity of APND in liver of grass carp (0.36 and 0.71 μg.L-1) was significantly higher than that of control (P <0.05 or P <0.01), and was significantly inhibited at 72 h (P <0.01) After 120 h, APND activity in each exposed group was significantly inhibited compared with the control group (P <0.05 or P <0.01). Overall ERND activity was induced. The GST activity showed an overall trend of being suppressed first. After exposure to 0.36 and 0.71 μg.L-1, the GST activity reached the highest value at 72 h, then slowly decreased with the prolongation of exposure time, and reached its peak at 168 h Was inhibited; 0.18μg.L-1 exposure group reached the maximum at 120 h, then decreased, 168 h GST activity and control group level. After exposure to endosulfan, grass carp liver cells significantly damaged DNA, TL and% TDNA increased with the increase of endosulfan concentration or exposure time, and the correlation was significant. Endosulfan can affect the phase Ⅰ and phase Ⅱ metabolic enzyme activities in the liver of grass carp and cause genetic damage to hepatocyte DNA.