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在患过敏性皮肤病患者的皮肤组织中,嗜硷细胞增多。嗜硷细胞中,有相当量的组织胺。有些人认为,过敏性皮肤病(风团、水泡等)的发生,与嗜硷细胞的颗粒溶解释放出组织胺有关。因此,近年来有人用观察抗原抗体反应时嗜硷细胞的脱粒现象,来判定抗原(过敏原)的性质。此即嗜硷细胞脱粒试验。嗜硷细胞脱粒试验所用的材料有:患者的血清,经稀释的抗原(药物或食物蛋白等),以及特制的家兔白细胞血沉棕黄层(含有较多的嗜硷细胞)。方法是先以火焰清洁玻片,待冷至微温时,在玻片上滴加新鲜配制的染液(5毫升纯乙醇内加0.6毫升中性红),置于空气中待干;用微吸管吸取0.005毫升患者血清滴在染液玻片上,加家兔白细胞血沉棕黄层,再加0.005毫升抗原,在空气中轻轻摇动玻片使之混合后,用周围涂以
In patients with allergic skin disease in the skin tissue, basophilic cells increased. Basophils, there is a considerable amount of histamine. Some people think that the occurrence of allergic skin diseases (wind groups, blisters, etc.), and the alkaloidal granules dissolved to release histamine. Therefore, in recent years, it has been used to determine the antigen (allergen) property by observing the threshing phenomenon of basophils during the antigen-antibody reaction. This is alkhyllithium threshing test. The basophilic cell degranulation test materials used: the patient’s serum, diluted antigen (drug or food protein, etc.), and a special rabbit white blood cell buffy coat (containing more basophils). Method is to first flame to clean the glass until cold to tepid, drip freshly prepared on the glass slide (5 ml of pure ethanol plus 0.6 ml of neutral red), placed in the air to be dry; with a micropipette 0.005 ml patient serum drops in the dye slides, add rabbit white blood cells buffy coat, add 0.005 ml of antigen, gently shake the slide in the air to make the mixture, with the surrounding coated