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目的:研究用微乳提取番石榴叶中槲皮素的方法。方法:考察油相、水、表面活性剂及助表面活性剂的比例,确定最佳微乳处方;用HPLC法测定微乳提取物中槲皮素的含量,并与溶剂提取法比较。结果:溶剂提取方法中,以甲醇提取得到的槲皮素含量最大,为4.72μg/mL;而在拟定的多种不同处方微乳的提取物中,有72%的处方所提取的槲皮素比甲醇的多;最佳微乳处方乙酸乙酯(油相)-30%异丙醇(水相)-RH60(表面活性剂)(油相∶水相=3∶7)提取的量是甲醇的两倍多,为10.43μg/mL。结论:与传统溶剂提取方法相比,微乳提取法能有效地提高番石榴叶中槲皮素的提取率。
Objective: To study the method of extracting quercetin from guava leaf with microemulsion. Methods: The ratio of oil phase, water, surfactant and cosurfactant was investigated to determine the optimal microemulsion prescription. The content of quercetin in the microemulsion was determined by HPLC and compared with the solvent extraction method. Results: In the solvent extraction method, the content of quercetin extracted by methanol was the highest, which was 4.72μg / mL. Among the extracts of many kinds of prescription microemulsions proposed, 72% of the quercetin The best microemulsion prescription Ethyl acetate (oil phase) - 30% isopropanol (water phase) -RH60 (surfactant) (oil phase: water phase = 3: 7) The amount extracted is methanol More than twice as much as 10.43 μg / mL. Conclusion: Compared with the traditional solvent extraction method, the microemulsion extraction method can effectively improve the extraction rate of quercetin in guava leaves.