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为了探讨强直性脊柱炎(AS)患者外周血白细胞中B细胞活化因子(BAFF)及其受体(BR3)的表达水平及其临床意义。采用流式细胞术检测24例AS患者、30名健康体检者外周血白细胞上BAFF、BR3的表达水平,并比较其与红细胞沉降率(ESR)、C反应蛋白(CRP)、免疫球蛋白(IgG、IgA、IgM)的相关性。结果显示,AS患者中BAFF在单核细胞上的表达水平、BR3在淋巴细胞上的表达水平明显高于正常对照组(P<0.01),两者之间明显正相关(r=0.588,P=0.003)。同时与健康人相比,AS患者的ESR、血清IgG、IgA、CRP的含量明显升高(P<0.05);AS患者的外周血单核细胞上BAFF的表达水平分别与血清IgG、IgA、CRP、ESR含量之间呈正相关(r=0.813,P=0.000;r=0.665,P=0.000;r=0.514,P=0.010;r=0.417,P=0.043)、外周血淋巴细胞上BR3的表达水平也分别与血清CRP、ESR水平之间明显正相关(r=0.672,P=0.000;r=0.416,P=0.043)。研究表明,AS患者外周血白细胞中BAFF及其受体BR3的表达水平异常增多,提示BAFF及其受体可能与AS的发病及疾病进程有关。
To investigate the expression of B-cell activating factor (BAFF) and its receptor (BR3) in peripheral leukocytes in patients with ankylosing spondylitis (AS) and its clinical significance. Flow cytometry was used to detect the expression of BAFF and BR3 on peripheral blood leukocytes from 24 AS patients and 30 healthy subjects. The levels of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), immunoglobulin (IgG) , IgA, IgM). The results showed that the expression of BAFF on monocytes and the expression of BR3 on lymphocytes in AS patients were significantly higher than those in normal controls (P <0.01), and the positive correlation was found between them (r = 0.588, P = 0.003). The levels of ESR, serum IgG, IgA and CRP in patients with AS were significantly higher than those in healthy subjects (P <0.05). The expression of BAFF in peripheral blood mononuclear cells of AS patients was significantly correlated with serum IgG, IgA, CRP (R = 0.665, P = 0.000; r = 0.514, P = 0.010; r = 0.417, P = 0.043). The expression level of BR3 on peripheral blood lymphocytes There was also a significant positive correlation between serum CRP and ESR levels (r = 0.672, P = 0.000; r = 0.416, P = 0.043). Studies have shown that peripheral blood leukocytes in patients with AS BAFF and its receptor BR3 abnormal expression increased, suggesting that BAFF and its receptors may be related to the pathogenesis of AS and disease progression.