X-ray diffraction pattes of graphite oxide (GO) are theoretically simulated as a function of the displacements of carbon atoms using the Debye-Waller factor in terms of the Warren-Bodenstein equation.The results demonstrate that GO has the turbostratically stacked structure.The high order (001) peaks gradually disappear with the increase in atomic thermal vibrations along c-axis while the (hk0) ones weaken for the vibrations along a-axis.When the displacement deviation ua =0.015 nm and uc =0.100 nm the computed result is consistent with the experimental measurements.