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目的 探讨人小细胞肺癌细胞株NCI H4 4 6的生长抑素受体体外调变的规律。方法 采用体外放射配基结合分析法 ,以12 5I标记的生长抑素类似物 (RC 16 0 )为配基 ,在与NCI H4 4 6细胞进行体外饱和及竞争结合实验的基础上 ,进行RC 16 0诱导下NCI H4 4 6细胞株的生长抑素受体内化及上调实验。结果 温育 15min时 ,内化组与对照组的内化率分别为 (2 2 .30± 2 .4 8) %和 (8.2 0± 3.2 8) % (P <0 .0 1) ,膜结合率分别为 (43.83± 3.97) %和 (12 .37± 1.6 1) % (P <0 .0 1)。低浓度的非标记RC 16 0暴露前 13h ,上调率增加缓慢 ,仅为 (2 0 .0 0± 1.30 ) % ,其后上调率迅速增加 ,并于暴露后 19h达到顶峰 [(76 .5 0± 2 .6 0 ) % ]。结论 RC 16 0可使NCI H4 4 6细胞表面生长抑素受体内化并上调 ,且具有一定的时相性
Objective To investigate the in vitro regulation of somatostatin receptor in human small cell lung cancer cell line NCI H4 4 6 . METHODS: In vitro radioligand binding assays were used. RC 125 was based on 12 5 I-labeled somatostatin analogue (RC 16 0 ) as a ligand and in vitro saturation and competition binding experiments with NCI H4 4 6 cells. 0 induced NCI H4 4 6 cell line somatostatin receptor internalization and upregulation experiments. Results After incubation for 15 min, the internalization rates of internalization group and control group were (22.30±2.88%) and (8.20±3.28%) (P < 0.01), respectively. The rates were (43.83 ± 3.97)% and (12.37 ± 1.61)% (P <0.01). At the low concentration of unlabeled RC 16 0 h before exposure, the rate of increase was slow, only (2 0 .0 ± 1.30) %, and the rate of up-regulation thereafter increased rapidly and peaked at 19 h after exposure [(76.5). ± 2 .6 0 ) % ]. Conclusion RC 16 0 can internalize the somatostatin receptor on NCI H4 4 6 cells and up-regulate it.