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本文介绍一种不经层析的血清双氢睾丸酮(DHT)和睾丸酮(T)放射免疫测定法(RIA)。以抗DHT抗血清为结合剂,利用特异性的T灭活剂,破坏血中存在的高浓度T分子活性,使DHT测定不受干扰;并利用抗DHT抗血清与T的交叉反应性作T测定。血清样品经乙醚提取,蒸干有机相加缓冲液重组,部分重组液直接作T-RIA,其余部分加T灭活剂反应15分钟,再经乙醚提取、蒸干,用缓冲液重组作DHT-RIA。本方法的提取回收率是94.5±4.0％(均数±标
This article describes a non-chromatographic serum DHT and testosterone radioimmunoassay (RIA). Anti-DHT antiserum as a binding agent, the use of specific T-inactivating agents, the destruction of the blood of high concentrations of T molecule activity, so that DHT determination without interference; and the use of anti-DHT antiserum and T cross-reactivity as T Determination. Serum samples were extracted with ether, evaporated to dryness and organic add buffer was recombined, part of the recombinant solution directly T-RIA, the rest of the T-activator reaction for 15 minutes, and then extracted with ether and evaporated to dryness, rebuilt with buffer for DHT- RIA. The extraction recovery of this method is 94.5 ± 4.0% (mean ± standard