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目的建立高效液相色谱法测定人血浆中头孢噻利血药浓度的方法。方法以甲硝唑为内标,色谱柱为ZORBAXExtend-C18(4.6 mm×250 mm,5μm),以乙腈-pH 6.5磷酸盐缓冲液(5.5∶94.5)为流动相,流速为1.0 mL.min-1,检测波长为254 nm,进样量为20μL,柱温为25℃。结果头孢噻利的血药浓度在1~200 mg.L-1内线性良好(r=0.999 9),定量限为1 mg.L-1;平均方法回收率为99.8%(RSD=1.53%);日内、日间RSD均<3%。结论本方法简便、灵敏、快速、准确,可用于头孢噻利的临床药动学研究和生物利用度研究。
Objective To establish a HPLC method for the determination of ceftibuten in human plasma. Methods Metronidazole was used as internal standard. The mobile phase consisted of ZORBAXExtend-C18 (4.6 mm × 250 mm, 5 μm) and acetonitrile-pH 6.5 phosphate buffer (5.5:94.5) with a flow rate of 1.0 mL · min- 1, the detection wavelength is 254 nm, the injection volume is 20 μL, the column temperature is 25 ℃. Results The concentration of ceftibrin in blood was 1 ~ 200 mg · L-1 (r = 0.999 9), the limit of quantification was 1 mg.L-1. The mean recovery was 99.8% (RSD = 1.53% ; Day, day RSD <3%. Conclusion The method is simple, sensitive, rapid and accurate and can be used in clinical pharmacokinetics and bioavailability studies of ceftitriane.